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KMID : 0545120080180030579
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 3 p.579 ~ p.584
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Evaluation of a Chromogenic Medium Supplemented with Glucose for Detecting Enterobacter sakazakii
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Song Kwang-Young
Hyeon Ji-Yeon
Shin Ho-Chul
Park Chan-Kyu
Choi In-Soo
Seo Kun-Ho
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Abstract
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A commercial chromogenic agar medium (DFI) was supplemented with glucose (mDFI) to enhance the specificity of Enterobacter sakazakii (E. sakazakii) detection. Escherichia vulneris (E. vulneris), a putative false-positive strain on the DFI medium, produces α-glucosidase. The enzyme α- glucosidase hydrolyzes a substrate, 5-bromo-4-chloro-3- indolyl-α,D-glucopyranoside (XαGlc), producing green colonies. E. sakazakii strains produced green colonies on both DFI and mDFI agar, whereas E. vulneris produced green colonies on DFI agar but small white colonies on mDFI agar. E. sakazakii and E. vulneris were also readily differentiated by colony color when the mixed culture of the two strains was plated on mDFI agar and incubated for 24 h at 37oC. The results indicate that the selectivity of the commercial chromogenic agar medium could be improved by a simple supplementation with glucose.
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KEYWORD
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Chromogenic agar, Enterobacter sakazakii, Escherichia vulneris, glucose
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